Pūtau Electrophoresis mo te SDS-PAGE me te Western Blot

Whakaahuatanga Poto:

Ko te DYCZ-24DN mo te electrophoresis pūmua, ko te DYCZ-40D mo te whakawhiti i te ngota ngota pūmua mai i te kiri ki te membrane penei i te membrane nitrocellulose i roto i te whakamatautau WesternBlot. I konei he huinga tino pai mo o taatau kaihoko ka taea te whakatutuki i te tono ka taea e te kaiwhakamatautau te whakamahi kotahi tank hei mahite hiko hiko, ka whakawhiti i tetahi kōwae hiko ki te mahi i te whakamatautau blotting e te taua tank DYCZ-24DN. Ko te mea e hiahia ana koe he punaha DYCZ-24DN me te kōwae DYCZ-40D Electrode ka taea e koe te huri tere me te ngawari mai i tetahi tikanga hiko ki tetahi atu.


Taipitopito Hua

Tohu Hua

Whakatakotoranga

Ahu (L×W×H)

140×100×150mm

Rahi Rahi (L×W)

75×83mm

Heru

10 nga puna me te 15 nga puna

Heru Matotoru

1.0mm me te 1.5mm (Paerewa)

0.75mm (Kōwhiringa)

Te maha o nga Tauira

20-30

Rōrahi pūreirei

400ml

Taumaha

1kg

Whakaahuatanga

Ko te DYCZ-24DN he pūtau hiko hiko (tank/chamber) mo te SDS-PAGE, PAGE Maori me etahi atu Protein electrophoresis. Ka taea e tenei pūtau te maka me te whakahaere i te reera ki te waahi kotahi. He maamaa me te motuhake he ngawari me te waatea ki te uta i nga tauira. Ko te tank he mea hanga ki te papanga polycarbonate kounga teitei, he tino roa, he maamaa hoki. Ma tenei tank marama he ngawari ki te mataki i te reera ka mahia te whakamatautau. Ko te DYCZ-24DN he hiko tango e ngawari ana mo te tiaki. Ko nga hiko he mea hanga e te konukawata parakore (≥99.95%) he hiko-waikura me te tu ki te pāmahana teitei.

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I muri i te electrophoresis gel, i runga i te whakaritenga whakamatautau, i etahi wa, me whakawhiti e te kaiwhakamatautau te kiri ki te tautoko totoka mo etahi atu tātaritanga. Ka kiia ko te whakamatautau blotting, he tikanga mo te whakawhiti pūmua, DNA, RNA ranei ki runga i te kawe. Ka mahia i muri i te electrophoresis gel, te whakawhiti i nga ngota ngota mai i te ra ki runga ki te membrane blotting. Whai muri i te murunga, ka kitea nga pūmua kua whakawhitia, te DNA, te RNA ranei ma te tae o te tae (hei tauira, te whakakikorua i te hiriwa o nga pūmua), te whakakitenga autoradiographic o nga ngota ngota reo irirangi (i mahia i mua i te para), te tapanga motuhake ranei o etahi pūmua, waikawa nucleic ranei. Ko te mea whakamutunga ka mahia ki nga paturopi, ki nga rangahau ranu ranei e herea ana ki etahi ngota ngota o te paraka anake, ka honoa he enzyme ki a ratou. Whai muri i te horoinga tika, ka kitea tenei mahi enzymatic (a, ko nga ngota ngota e rapuhia ana e tatou i roto i te para) ka kitea ma te heki me te tauhohenga tika, ka puta he putea tae ki runga i te puru, he tauhohenga chemiluminescent ranei kua rehitatia e te kiriata whakaahua.

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Mo te tuku hiko mo tenei pūtau hiko kaera poutū, ka tūtohu matou ki tetahi o te mana hiko hiko te tauira DYY-6C.

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Taupānga

Mo te SDS-PAGE, Native PAGE electrophoresis me te whakawhiti i te ngota ngota pūmua mai i te kiri ki te membrane.

Āhuahira

Ko te DYCZ-24DN mini poutū pūtau electrophoresis gel mo te SDS-PAGE, Native PAGE electrophoresis e whai ake nei:

He mea hanga mai i te polycarbonate maramara teitei, he ataahua, he roa, he ngawari ki te mataki;

• Ma te whakarewa i te reera ki te tuunga taketake, ka taea te maka me te whakahaere i te reera ki te waahi kotahi, he ngawari, he watea hoki ki te hanga gels, me te whakaora i to wa utu nui;

• Ka taea e te hoahoa anga poro motuhake te whakatika i te ruuma reera;

• I whakakikoruatia te taika parapara e mau ana i nga hiko konukawata parakore;

• He ngawari me te watea ki te taapiri i nga tauira;

Ka taea ki te rkotahi te ra, e rua ranei nga ra i te wa kotahi;

• Whakaorangia te otinga parepare;

• Ko te hoahoa motuhake o te taika kia karo i te parepare me te rire reera;

Ko nga electrodes tango, he ngawari ki te pupuri me te ma;

• Whakaweto-aunoa ina whakatuwheratia te taupoki;

Ko te waahanga hiko, e kiia ana ko te tinana tautoko mo te whakawhiti, te huihuinga hiko ranei he waahanga matua mo te punaha blotting DYCZ-40D. Kei roto i nga waahanga tae whero me te pango me nga hiko whero me te pango hei whakarite i te whakatakotoranga tika o te gel i te wa o te whakawhitinga, me te hoahoa pai e ngawari ana te whakauru me te tango i nga riipene pupuri reera mai i te tinana tautoko mo te whakawhiti (whakahuihui hiko).

ae26939e xz


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