Te Whakaritenga Tauira me teUta ana
Na te whakamahi i te punaha kaapiri tonu me te kore e whakakao i te reera i roto i te nuinga o nga keehi, me tika te kukū me te iti o te rōrahi o nga tauira. Whakamahia apaipakia āta tāpirihia te tauira, me te 5-10 μg mo ia puna, hei karo i te heke nui o te taumira. Inautaina tauira, me whakawetohia te hiko hiko. Ko te tauira he tae tohu (0.025% bromophenol kahurangi, karaka ranei) me te sucrose (10-15%), glycerol ranei (5-10%) hei whakanui ake i tona kiato, kia aro te tauira, kia kore ai te whakamaarama. Heoi ano, i etahi wa ka puta he riipene ahua-U i etahi wa, ka taea te karo ma te whakamahi i te 2.5% Ficoll (polyvinylpyrrolidone).
Electrophoresis
Ko te ngaohiko mo te electrophoresis he 5-15 V/cm, i te nuinga o te 10 V/cm. Mo te wehenga o nga ngota ngota nui, me iti ake te ngaohiko, te tikanga kaua e neke ake i te 5 V/cm.
Te whakapouri
Ko te ethidium bromide (EB) te waikano rewharewha e whakamahia ana mo te whakapouri ki te maataki i nga here DNA i roto i te kaera agarose. Ka taea e te EB te whakauru ki waenga i nga takirua taketake o nga ngota ngota DNA, ka herea te EB ki te DNA. Ko te whakaurunga o te rama ultraviolet 260 nm e te DNA ka whakawhitia ki te EB, a, ko te EB herea e tuku ana i te 590 nm i roto i te rohe whero-karaka o te awhiowhio marama kitea. Ma te whakapouri i te reera i roto i te otinga 1 mmol/L MgSO4 mo te 1 haora ka taea te whakaiti i te rewharewha o te papamuri na te EB kore i herea, ka taea te kite i te iti o te DNA.
He maha nga painga o te waikano EB: he ngawari ki te whakamahi, kaore e pakaru i te waikawa karihi, he nui te tairongo, ka taea te whakapouri i te DNA me te RNA. Ka taea te taapiri EB ki te tauira me te whai ma te whakamahi i te whakauru UV i nga wa katoa. I muri i te paru, ka taea te tango i te EB ma te tango i te n-butanol.
Heoi, ko te tae EB he mutagen kaha, a me mau nga whakatupato, penei i te mau karapu polyethylene, i te wa e whakahaere ana. Ko te karaka acridine hoki he waikano e whakamahia nuitia ana na te mea ka taea e ia te wehewehe i waenga i te whenu kotahi me te whenu-rua waikawa karihi (DNA, RNA). E whakaatu ana i te korikori kaakaariki (530 nm) mo nga waikawa karihi paerua-rua me te rewharewha whero (640 nm) mo nga waikawa nucleic kotahi. I tua atu, ka taea te whakamahi etahi atu tae penei i te methylene blue, methylene green, me te quinoline B mo te whakapouri.
Ko Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he mea motuhake ki te hanga taputapu hiko mo te neke atu i te 50 tau me o taatau ake roopu hangarau ngaio me te pokapū R&D. Kei a matou te raina whakaputa pono me te whakaoti mai i te hoahoa ki te tirotiro, me te whare putunga, me te tautoko hokohoko. Ko o tatou hua matua ko te Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.
Kei te rapu hoa taatau inaianei, kei te powhirihia nga tank electrophoresis OEM me nga kaitoha.
Mena kei a koe etahi mahere hoko mo a maatau hua, kaua koe e mangere ki te whakapiri mai ki a maatau. Ka taea e koe te tuku karere mai ma te imeera[kua tiakina te imeera]ranei[kua tiakina te imeera], waea mai ranei ki a matou i +86 15810650221, taapirihia ranei a Whatsapp +86 15810650221, Wechat ranei: 15810650221.
Tena Matawaihia te waehere QR hei taapiri atu ki a Whatsapp, WeChat ranei.
Wā tuku: Tihema-20-2023