Te Whakaritenga o te Aarara Akara mo te Electrophoresis
Tuhipoka: Me mau i nga wa katoa nga karapu tuku!
Nga Tohutohu-a-Taahiraa
Paura Paura Agarose:uhe pepa pauna me te pauna hiko hei ine i te 0.3g o te paura agarose (i runga i te punaha 30ml).
Te whakarite i te TBST Buffer:pwhakatikahia te 30ml o te 1x TBST parepare i roto i te ipu Erlenmeyer 100ml.
Whakarewa i te Paura Agarose:pta tatou i te paura agarose ki roto i te parapara TBST ka wiri pai.Whakanohoia ki rotoki te ngaruiti me te wera (te nuinga mo te 50 hēkona, ka uhia ki te pepa konumohe) kia rewa katoa.
Te whakamatao me te taapiri i te Nuclease:uhe karapu hei tango i te ranunga mai i te ngaruiti ka waiho kia whakamatao paku ki te wai matao kia mahana (tata ki te 60°C). Taapirihia te 2µl o te nuclease (whakakapi i te Eb) i te wa e wiri ana kia uru pai.
Te whakarite i te Mold Gel:
- Chiroki ka maroke tepaepae reera me te taputapu whakarewa reerao te tank electrophoresis.
- Pmirohia te reerapaepaeki roto i te taika o roto ka kuhu i te heru ki te tuunga tuturu.
- Whakaranuhia te wairewa ira agarose, whakamatao ki te 65°C, ka ata ringihia ki runga i tepaepae reerai roto i tetaputapu whakarewa reera, ka horahia kia ata noho kia puta he paparanga reera ki runga i te pereti karaihe.
- Tukua kia noho ki te pāmahana rūma tae noa ki te tino pakari o te ra.
- Me ata tango te heru poutū ka tango i te riipene.
- Whakanohoia te reerapaepaeki roto i te tank electrophoresis.
He mea nui: Me whakarite karekau he mirumiru hau i te waahi niho heru. Ko te mata o te wai kia maeneene me te kore he ripple.
Te whakahaere i te Gel
Te uta i te Gel
I muri i te whakamaroketanga o te ra, tuu ki roto i te tank electrophoresis ka ringihia te parai hiko kia rukuhia te reera.
Te Whakarite Tauira
- Tangohia te tohu me te uta i te parapara mai i te pouaka whakamātao.
- Taapirihia te 6µl o te parai uta ki nga tauira ka uru pai.
- Ma te whakamahi i te micropipette, utaina atahia nga tauira ki roto i nga puna nui o te reera (kia tupato kei werohia te reera, kaua hoki e tukuna te katoa o te rōrahi kia kore ai te mirumiru hau).
- Utaina te tohu ki tetahi o nga puna iti (maharatia tona turanga).
Tīmatahia te Electrophoresis
- Hipokina te tank electrophoresis ka timata i te electrophoresis i muri tonu i te utaina o te ra.
- Whakaritea te ngaohiko ki te 60-100V. Ka heke nga tauira mai i te hiko kino (pango) ki te hiko pai (whero).
- Ko te ngaohiko teitei ake ka whakapoto i te awhe wehewehenga whai hua o te kaera agarose.
- Kati te electrophoresis ina tae te tae puru bromophenol ki te 1cm mai i te tapa o raro o te pereti reera.
Te Mataki i nga Hua
I muri i te wehenga o nga here, whakamutua te electrophoresis, tangohia te kiri, ka kitea tika me te whakaahua.
Whakamahia he punaha whakaahua reera hei whakaahua me te mataki i nga roopu (tirohia mena he roopu mo te tohu, tauira ranei).
I muri i te whiwhinga o to mapi roopu reera, kimihia te tohu. I runga i te tohu, ka taea e koe te whakatau i nga roopu whaainga!
Ko Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) kua hangaia nga hua hiko mo te neke atu i te 50 tau. Ka whakaatuhia e matou a maatau punaha hiko whakapae mo koneikia maurielectrophoresis.Kōwhiringa punaha hiko whakapaemai i Beijing liuyi mo to whakamatautau ira electrophoresis hei awhina i to rangahau i te umanga hangarau koiora.
Ka whakaekea e matou etahi tauira rereke o nga taika hiko hiko whakapae mo te maka me te rere itiagarosekare ki te nuiagarosengongo
He Aratohu Whiriwhiringa mo te Waeine Electrophoresis Gel Rumaki Whakapae
Ko Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he mea motuhake ki te hanga taputapu hiko mo te neke atu i te 50 tau me o taatau ake roopu hangarau ngaio me te pokapū R&D. Kei a matou te raina whakaputa pono me te whakaoti mai i te hoahoa ki te tirotiro, me te whare putunga, me te tautoko hokohoko. Ko o tatou hua matua ko te Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Ka tukuna ano e matou nga taputapu taiwhanga penei i te taputapu PCR, te whakaranu vortex me te centrifuge mo te taiwhanga.
Mena kei a koe etahi mahere hoko mo a maatau hua, kaua koe e mangere ki te whakapiri mai ki a maatau. Ka taea e koe te tuku karere mai ma te imeera[kua tiakina te imeera]ranei[kua tiakina te imeera], waea mai ranei ki a matou i +86 15810650221, taapirihia ranei a Whatsapp +86 15810650221, Wechat ranei: 15810650221.
Tena Matawaihia te waehere QR hei taapiri atu ki a Whatsapp, WeChat ranei.
Wā tuku: Akuhata-09-2024