He aha te tukanga paihikara waiariki i roto i te PCR?

Ko te Polymerase Chain Reaction (PCR) he tikanga koiora irapoi hei whakanui ake i nga maramara DNA. Ka taea te whakaaro he ahua motuhake o te tukurua DNA i waho o te rauropi ora. Ko te ahuatanga matua o te PCR ko tona kaha ki te whakanui i te nui o te DNA.

Tirohanga o te Hurihanga Tauhohenga mekameka Polymerase

Whakaahua mai i a Bioninja

He aha te tukanga paihikara waiariki i roto i te PCR?

Ko te PCR e toru nga waahanga matua:

1. Te whakahekenga o te DNA tauira: Ka whakamahana te DNA tauira ki te 93°C mo tetahi wa roa, ka wehe te DNA whenu-rua ki nga whenu kotahi, ka whakareri mo te here tuatahi i te huringa tauhohenga ka whai ake.

2. Te Whakapiri i te DNA Tauira me nga Panui: I muri i te denaturation, ka whakahekehia te pāmahana ki te 55°C, ka taea te here o nga mea tuatahi ki nga raupapa taapiri o te DNA tauira whenu kotahi.

3. Toronga o nga Primers: I te 72°C, ka toroa te matatini tauira-primer DNA ma te awhina o te DNA polymerase (pēnei i te Taq DNA polymerase), ma te whakamahi i nga dNTP hei taputapu hei whakahiato i tetahi whenu taapiri hou i runga i te tauira DNA.

Beijing LIUYI Mīhini PCR

Ma te tukurua i nga tikanga whakaheke, whakaene, me te toronga, ka nui ake nga "nga whenu tukurua-a-waa" ka puta, ka waiho hei tauira mo nga huringa ka whai ake. He 2 ki te 4 meneti te roa o ia huringa, ka taea te whakanui ake i te ira i whäia i roto i te 2 ki te 3 haora.

Ko Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he mea motuhake ki te hanga taputapu hiko mo te neke atu i te 50 tau me o taatau ake roopu hangarau ngaio me te pokapū R&D. Kei a matou te raina whakaputa pono me te whakaoti mai i te hoahoa ki te tirotiro, me te whare putunga, me te tautoko hokohoko. Ko o tatou hua matua ko te Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Ka tukuna ano e matou nga taputapu taiwhanga penei i te taputapu PCR, te whakaranu vortex me te centrifuge mo te taiwhanga.

Mena kei a koe etahi mahere hoko mo a maatau hua, kaua koe e mangere ki te whakapiri mai ki a maatau. Ka taea e koe te tuku karere mai ma te imeera[kua tiakina te imeera]ranei[kua tiakina te imeera], waea mai ranei ki a matou i +86 15810650221, taapirihia ranei a Whatsapp +86 15810650221, Wechat ranei: 15810650221.

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