He uaua ki a koe ki te whakareri i te rae agarose?Kia whai aketo tatou kaitoi taiwhanga ki te whakarite i te reera.
Ko te whakaritenga o te gel agarose ko nga waahanga e whai ake nei:
Paura Paura Agarose
Paunatia te nui o te paura agarose kia rite ki te kukū e hiahiatia ana mo to whakamatautau. Ko nga kohinga agarose noa mai i te 0.5% ki te 3%. Ka whakamahia nga kohinga teitei ake mo te wehe i nga ngota ngota DNA iti, engari ko nga kohinga iti ake mo nga ngota nui ake.
Te Whakareri i te Otinga Buffer
Tāpirihia te paura agarose ki tētahi pūrei electrophoresis tika, pēnei i te 1× TAE, 1× TBE rānei. Me ōrite te rōrahi o te pūreirei ki te rōrahi o te rā e hiahiatia ana mō tō whakamātau.
Te whakakore i te Agarose
Whakawerahia te ranu kia rewa me te ranu kia rewa rawa te agarose. Ka taea tenei ma te whakamahi i te ngaruiti, te pereti wera ranei. Pokarekaretia te otinga kia kore ai e koropupuke. Ko te wairewa agarose kia maamaa me te kore he matūriki e kitea.
Te whakamatao i te Rongoa Agarose
Tukua kia whakamatao te wairewa agarose kua wera ki te 50-60°C. Whakakorikorihia te otinga i te wa e whakamahana ana kia kore ai e pakari te wa o mua.
Taapiri Waiwawa Nucleic Stain (Kōwhiringa)
Mena kei te pirangi koe ki te whakaata i te DNA, i te RNA ranei i roto i te reera, ka taea e koe te taapiri i te waiu waikawa karihi, penei i te GelRed, te ethidium bromide ranei, i tenei wa. I te wa e whakahaere ana i enei piro, mau karapu me te mahi tupato, he paitini.
Maka ana te Gel
Ringihia te wairewa agarose kua whakamatao ki roto i te pokepokea ai te reera electrophoresis. Whakauruhia he heru hei hanga i nga puna tauira, kia mau te heru, kia taurite te tohatoha o te otinga ki roto i te pokepokea ai.
Whakakotahitanga Gel
Whakaaetia te ra kia pakari i te pāmahana rūma, he 20-30 meneti te roa i runga i te kukū me te matotoru o te reera.
Rte tango i te Heru
Kia tino pakari te kiri, ata tango i te heru kia kitea nga puna tauira. Whakanohoia te reera me te pokepokea ki roto i te ruuma electrophoresis ka hipoki ki te rahinga tika o te putunga electrophoresis, kia tino rukuhia te reera.
Te whakarite mo te Electrophoresis
I muri i te reri o te ra, utaina o tauira ki roto i nga puna, ka haere ki te whakamatautau electrophoresis.
Mena kei a koe etahi take mo te whakarite gel, tena koa whakapiri mai ki a maatau. Kei a matou nga tohunga hangarau taiwhanga e waatea ana ki te korero ki a koe.
Kei te harikoa matou ki te whakapuaki i etahi korero pai: kei te whakatairangahia to maatau waka hiko whakapae whakapae DYCP-31DN i tenei wa, whakapā mai mo etahi atu korero inaianei!
Ko Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he mea motuhake ki te hanga taputapu hiko mo te neke atu i te 50 tau me o taatau ake roopu hangarau ngaio me te pokapū R&D. Kei a matou te raina whakaputa pono me te whakaoti mai i te hoahoa ki te tirotiro, me te whare putunga, me te tautoko hokohoko. Ko o tatou hua matua ko te Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Ka tukuna ano e matou nga taputapu taiwhanga penei i te taputapu PCR, te whakaranu vortex me te centrifuge mo te taiwhanga.
Mena kei a koe etahi mahere hoko mo a maatau hua, kaua koe e mangere ki te whakapiri mai ki a maatau. Ka taea e koe te tuku karere mai ma te imeera[kua tiakina te imeera]ranei[kua tiakina te imeera], waea mai ranei ki a matou i +86 15810650221, taapirihia ranei a Whatsapp +86 15810650221, Wechat ranei: 15810650221.
Tena Matawaihia te waehere QR hei taapiri atu ki a Whatsapp, WeChat ranei.
Te wa tuku: Oketopa-15-2024