He aha te Cellulose Acetate Membrane Electrophoresis?
Ko te Cellulose Acetate Membrane Electrophoresis tetahi momo tikanga electrophoresis e whakamahi ana i te membrane cellulose acetate hei papaaho tautoko mo nga whakamatautau.
Ko te Cellulose Acetate he ahua o te acetate o te cellulose he acetylated mai i te cellulose's hydroxyl. I te wa e whakarewahia ana i roto i te wairewa pararopi penei i te acetone, ka taea te whakakikorua ki te kiriata riterite me te pai me nga pores moroiti. Ko te matotoru o te kiriata kei te 0.1-0.15mm e tika ana mo te whakamatautau.
Ko nga painga o te membrane cellulose acetate
Ki te whakataurite ki te pepa fliter, kei a ia nga painga e whai ake nei:
1.Painga pai o te wehenga. Mo nga tauira pūmua, he iti noa te kiri o te acetate cellulose, i muri i te tae, ka taea te whakakore i te tae o te papamuri. He tino marama nga roopu waikano, na reira ka whakatairanga i te tika mo te whakatau ine.
2.Fast me te whakaora wa. Ko te cellulose acetate membrane he iti ake te hydyophilic i te pepa fliter, na te mea he iti ake te mahi electro-osmosis i te pepa fliter, a ko te hiko hiko e whakahaerehia ana e nga tauira ka taea te poto i te wa wehenga me te iti ake o te wa electrophoresis. Ko te tikanga, ko te wa electrophoresis membrane cellulose acetate e pā ana ki te 45-60minutes. Ko te tukanga katoa o te electrophoresis tae atu ki te tae me te whakakoi me te 90 meneti noa iho.
3.High sensitivity me te iti o nga tauira hei whakamahi. Me 2 μL serum anake i roto i te electrophoresis pūmua serum.
4.Wide tono. Ko etahi o nga pūmua kaore i te ngawari ki te wehe i runga i te electrophoresis pepa, penei i te Alpha-fetoprotein, te enzyme bacteriolytic, te insulin, me te histone me etahi atu. Engari he pai ake te whakamahi i te cellulose acetate membrane electrophoresis hei wehe.
5.Easy ki te pupuri me te ine. Whai muri i te tae o nga tauira electrophoresis membrane cellulose acetate, rukuhia nga tauira ki te otinga whakauru o te waikawa ahetiki me te waiwaro hei hanga i tetahi pereti maroke maramara ka tiakina mo te wa roa.
Na te mea he ngawari te whakahaere i te electrophoresis membrane cellulose acetate, inaianei kua whakamahia nuitia ki te tātari me te whakamatautau i te pūmua plasma, lipoprotein, glycoprotein, Alpha-fetoprotein, enzyme, polypeptide, waikawa nucleic me etahi atu biomacromolecule.
Whakaritenga mo te Cellulose Acetate Membrane Electrophoresis
Ko nga taputapu e whakamahia ana ko te iti o te ngaohiko hiko, he hiko hiko whakapae. Ko te papanga tautoko ko te membrane cellulose acetate.
Ko nga reagents:
1.pH8.6 barbitol wairewa parepare: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain:Ponceau S, Trichloroacetic;
3.TBS/T or PBS/T: 95% ethanol, waikawa acetic glacial;
4.Cleaning Solution: waiwaro anhydrous, waikawa acetic glacial.
Beijing Liuyi Biotechnology Co., ltd he pūnaha pakeke mo cellulose acetate membrane electrophoresis. Ko te tauira DYCP-38C he ruma ngawari mo te pepa me te hiko hiko, me te cellulose acetate membrane electrophoresis. Ko te tinana matua kua whakarewahia, kaore he ahuatanga leakage. Ko te tauira hiko DYY-2C, DYY-6C, DYY-6D, DYY-8C, DYY-10C me DYY-12 ka taea te tuku mana mo DYCP-38C. Ko te tikanga, e pai ana te kaihoko ki te kowhiri i te tauira DYY-6C hei taonga hiko mo DYCP-38C.
Ko te hua e tika ana mo te tank electrophoresis DYCP-38C, ka tukuna ano e Liuyi te membrane cellulose acetate. E toru nga rahi noa o te membrane, 7 * 9cm, 2 * 8cm me te 12 * 8cm, a ka taea e matou te whakarato i te rahi o te cellulose acetate membrane rite ki o hiahia.
Nga korero mo te whakamatautau:
1.Utaina te tauira ki te taha taratara: 1-2UL he pai
2.Constant current electrophoresis: kaha o nāianei 0.4～0.6m A/cm
3.pH8.6 wairewa parepare barbitol:Te kaha katote 0.06
4.Wai tae: 5 meneti nui
5.Preservation: hoatu te mapi electrtophoresis maroke ki roto i te otinga horoi mo te 10-15 meneti, ka tango atu ka mau ki runga i te karaihe ma, i muri i te maroke, ka waiho hei mahere kiriata marama.
He nui ake i te 50 nga tau o te waitohu a Liuyi i Haina ka taea e te kamupene te whakarato hua pumau me te kounga teitei puta noa i te ao. Na te whanaketanga o nga tau, he pai ki a koe!
Mo etahi atu korero mo matou, tena koa whakapā mai ma te imeera[kua tiakina te imeera], [kua tiakina te imeera].
Wā tuku: Apr-01-2022