He aha te Cellulose Acetate Membrane Electrophoresis?
Ko te Cellulose Acetate Membrane Electrophoresis tetahi momo tikanga electrophoresis e whakamahi ana i te membrane cellulose acetate hei papaaho tautoko mo nga whakamatautau.
Ko te Cellulose Acetate he ahua o te acetate o te cellulose he acetylated mai i te cellulose's hydroxyl.I te wa e whakarewahia ana i roto i te wairewa pararopi penei i te acetone, ka taea te whakakikorua ki te kiriata riterite me te pai me nga pores moroiti.Ko te matotoru o te kiriata kei te 0.1-0.15mm e tika ana mo te whakamatautau.
Ko nga painga o te membrane cellulose acetate
Ki te whakataurite ki te pepa fliter, kei a ia nga painga e whai ake nei:
1.Painga pai o te wehenga.Mo nga tauira pūmua, he iti noa te kiri o te acetate cellulose, i muri i te tae, ka taea te whakakore i te tae o te papamuri.He tino marama nga roopu waikano, na reira ka whakatairanga i te tika mo te whakatau ine.
2.Fast me te whakaora wa.Ko te cellulose acetate membrane he iti ake te hydyophilic i te pepa fliter, na te mea he iti ake te mahi electro-osmosis i te pepa fliter, a ko te hiko hiko e whakahaerehia ana e nga tauira ka taea te poto i te wa wehenga me te iti ake o te wa electrophoresis.Ko te tikanga, ko te wa electrophoresis membrane cellulose acetate e pā ana ki te 45-60minutes.Ko te tukanga katoa o te electrophoresis tae atu ki te tae me te whakakoi me te 90 meneti noa iho.
3.High sensitivity me te iti o nga tauira hei whakamahi.Me 2 μL serum anake i roto i te electrophoresis pūmua serum.
4.Wide tono.Ko etahi o nga pūmua kaore i te ngawari ki te wehe i runga i te electrophoresis pepa, penei i te Alpha-fetoprotein, te enzyme bacteriolytic, te insulin, me te histone me etahi atu. Engari he pai ake te whakamahi i te cellulose acetate membrane electrophoresis hei wehe.
5.Easy ki te pupuri me te ine.Whai muri i te tae o nga tauira electrophoresis membrane cellulose acetate, rukuhia nga tauira ki te otinga whakauru o te waikawa ahetiki me te waiwaro hei hanga i tetahi pereti maroke maramara ka tiakina mo te wa roa.
Na te mea he ngawari te whakahaere i te electrophoresis membrane cellulose acetate, inaianei kua whakamahia nuitia ki te tātari me te whakamatautau i te pūmua plasma, lipoprotein, glycoprotein, Alpha-fetoprotein, enzyme, polypeptide, waikawa nucleic me etahi atu biomacromolecule.
Whakaritenga mo te Cellulose Acetate Membrane Electrophoresis
Ko nga taputapu e whakamahia ana ko te iti o te ngaohiko hiko, he hiko hiko whakapae.Ko te papanga tautoko ko te membrane cellulose acetate.
Ko nga reagents:
1.pH8.6 barbitol wairewa parepare: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain:Ponceau S, Trichloroacetic;
3.TBS/T or PBS/T: 95% ethanol, waikawa acetic glacial;
4.Cleaning Solution: waiwaro anhydrous, waikawa acetic glacial.
Beijing Liuyi Biotechnology Co., ltd he pūnaha pakeke mo cellulose acetate membrane electrophoresis.Ko te tauira DYCP-38C he ruma ngawari mo te pepa me te hiko hiko, me te cellulose acetate membrane electrophoresis.Ko te tinana matua kua whakarewahia, kaore he ahuatanga leakage.Ko te tauira hiko DYY-2C, DYY-6C, DYY-6D, DYY-8C, DYY-10C me DYY-12 ka taea te tuku mana mo DYCP-38C.Ko te tikanga, e pai ana te kaihoko ki te kowhiri i te tauira DYY-6C hei taonga hiko mo DYCP-38C.
Ko te hua e tika ana mo te tank electrophoresis DYCP-38C, ka tukuna ano e Liuyi te membrane cellulose acetate.E toru nga rahi noa o te membrane, 7 * 9cm, 2 * 8cm me te 12 * 8cm, a ka taea e matou te whakarato i te rahi o te cellulose acetate membrane rite ki o hiahia.
Nga korero mo te whakamatautau:
1.Utaina te tauira ki te taha taratara: 1-2UL he pai
2.Constant current electrophoresis: kaha o nāianei 0.4～0.6m A/cm
3.pH8.6 wairewa parepare barbitol:Te kaha katote 0.06
4.Wai tae: 5 meneti nui
5.Preservation: hoatu te mapi electrtophoresis maroke ki roto i te otinga horoi mo te 10-15 meneti, ka tango atu ka mau ki runga i te karaihe ma, i muri i te maroke, ka waiho hei mahere kiriata marama.
He nui ake i te 50 nga tau o te waitohu a Liuyi i Haina ka taea e te kamupene te whakarato hua pumau me te kounga teitei puta noa i te ao. Na te whanaketanga o nga tau, he pai ki a koe!
Mo etahi atu korero mo matou, tena koa whakapā mai ma te imeera[kua tiakina te imeera], [kua tiakina te imeera].
Wā tuku: Apr-01-2022